What is the CMV promoter?

The CMV promoter is a commonly used promoter for the production of high level recombinant protein in mammalian cells17. However, the expression level of the transgene driven by CMV promoter decreases with extended culture times because of transcriptional silencing, which is associated with DNA methylation18, 19.

Is CMV promoter constitutive expression?

A variety of cellular and viral constitutive promoters have been used in expression vectors to introduce exogenous genes into cells. These promoters have distinct advantages; for example, cytomegalovirus (CMV) early enhancer/promoter directs a high level of transient gene expression in many types of cells [1]–[4].

How long is CMV promoter?

Promoters are about 100 to 1000 base pairs long and found upstream of their target genes.

What is CMV plasmid?

General description. PSF-CMV-AMP – CMV PROMOTER PLASMID is a versatile cloning plasmid for the expression of genes in mammalian cells with an ampicillin resistance cassette. Promoter Expression Level: The plasmid vector contains the mammalian CMV promoter to drive gene expression.

Where does the CMV promoter come from?

Due to strong expression and compatibility with numerous cell types, promoters derived from the enhancer region of the cytomegalovirus (CMV) immediate early (IE) promoter are among the most common for general expression vectors.

Is CMV a DNA virus?

Cytomegalovirus (CMV) is a double-stranded DNA virus and is a member of the Herpesviridae family.

What is UBC promoter?

The promoter of the polyubiquitin C gene (UBC) contains putative heat shock elements (HSEs) which are thought to mediate UBC induction upon stress. However, the mapping and the functional characterization of the cis-acting determinants for its up-regulation have not yet been addressed.

Is CMV a strong promoter?

While there is cell type to cell type variability for all the promoters, CMV promoter is the most variable, being very strong in some cell types (e.g., 293T and CMMT) and rather weak in others (e.g., MRC5 and MSC).

How do you induce CMV promoter?

While acrolein was ineffective, hydrogen peroxide slightly (50 %) stimulated the CMV promoter. In contrast, HNE had a strong, up to 3-fold, enhancing effect on the CMV-1 promoter within four as well as after 24h of treatment. The most effective was the treatment with 24 microM HNE.

What is CMV GFP?

CMV-GFP lentivirus Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm).

What is a CMV vector?

Abstract. Cytomegalovirus (CMV) has a number of interesting properties that qualifies it as a vector for gene transfer. Especially appealing is the ability of the CMV genome to persist in hematopoietic progenitor cells and the packaging capacity of the viral capsid that accommodates a DNA genome of 230 kbp.

Is CMV genetic?

Genomic complexity of human CMV is one of the largest among various DNA viruses. With such genome complexity and widespread distribution, even a minimal frequency of mutation will be enough to create a complicated genetic heterogeneity in this virus.

How can I get the human cytomegalovirus (CMV) annotated DNA sequence?

Human cytomegalovirus (CMV) immediate early enhancer and promoter. To obtain this annotated DNA sequence with restriction sites, please download SnapGene or the free SnapGene Viewer. Your time is valuable! × Please choose an application for opening sequence files.

Is the CMV vector available from Addgene?

This page is informational only – this vector is NOT available from Addgene – please contact the manufacturer for further details. This vector is NOT available from Addgene. Human cytomegalovirus (CMV) immediate early enhancer and promoter.

How does recrecombinant cytomegalovirus replicate in Human Foreskin Fibroblasts?

Recombinant virus with the simian CMV counterpart replicated efficiently in human foreskin fibroblasts, as well as wild-type virus. In contrast, replacement with the murine CMV counterpart caused inefficient MIE gene transcription, RNA splicing, MIE and early viral gene expression, and viral DNA replication.

What is the role of the cis-acting element in viral replication?

We determined the role of the cis-acting element in viral replication by comparing recombinant viruses with the cis-acting element replaced with other sequences. Recombinant virus with the simian CMV counterpart replicated efficiently in human foreskin fibroblasts, as well as wild-type virus.